AceSeq Fungi/Yeast Genomic DNA Isolation Kit

The AceSeq Fungi/Yeast Genomic DNA Purification Kit is designed for efficient and high-yield purification of high-molecular-weight DNA from yeast and other fungi. Genomic DNA is efficiently extracted from the cells by a combination of heat treatment, detergents and Bead Tubes (provided). An optional lyticase treatment allows for improved DNA yields with certain fungal and yeast species. The protocol involves nonenzymatic cell lysis at 65C, followed by removal of protein by precipitation, and nucleic acid precipitation and resuspension. No lyticase, proteolytic enzymes, or bead-beating are used in the procedure. Protocol time is typically less then 30 minutes. Recovered genomic DNA is of excellent yield and purity for any downstream application including PCR, qPCR, Restriction Fragment Length Polymorphism (RFLP), Amplified Fragment Length Polymorphism (AFLP), sequencing, SNP analysis and more.