EnzyChrom Nitric Oxide Synthase Inhibitor Screening Kit

Application

• HTS for inhibitor screening and evaluation of nitric oxide synthase inhibitors.

Key Features

• High-throughput. Homogenous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling system.
• Rapid and reliable. Can be completed in less than 3 hours if assay performed at 37C.

Method

• OD540nm

Samples

• Nitric Oxide Synthase

Species

• All

Size

• 100 tests

Shelf Life

• 6 months

More Details

• Nitric oxide (NO) is a reactive radical that plays an important role in many key physiological functions. NO is the oxidation product of arginine by nitric oxide synthase (NOS) and is involved in host defense development, activation of regulatory proteins and direct covalent interaction with functional biomolecules. Inhibition of NOS has the potential to produce diverse biological effects, particularly in the cardiovascular system. Simple, direct and non-radioactive procedures for measuring NOS are becoming popular in research and drug discovery. BioAssay Systems EnzyChrom Nitric Oxide Synthase Inhibitor Assay Kit involves two steps: a NOS reaction step during which NO is produced followed by an NO detection step. Since the NO generated by NOS is rapidly oxidized to nitrite and nitrate, the NO production is measured following reduction of nitrate to nitrite using an improved Griess method.