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TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

TUNEL In Situ Apoptosis Kit (HRP-DAB Method)

Item no.	E-CK-A331-50
Manufacturer	Elabscience
Amount	50 Assays
Quantity options	100 Assays 20 Assays 50 Assays
Category	Other Assays & Kits Detection Kits
Type	Kit
Specific against	other
Citations	https://translational-medicine.biomedcentral.com/articles/10.1186/s12967-022-03746-4 / https://www.mdpi.com/2073-4409/11/19/2971 / https://europepmc.org/article/pmc/pmc9403485?javascript_support=no / https://www.ncbi.nlm.nih.gov/pmc/articles/pmc7955787/ / https://onlinelibrary.wiley.com/doi/abs/10.1111/jcmm.16197 / https://www.sciencedirect.com/science/article/pii/S156757692100521X / https://onlinelibrary.wiley.com/doi/abs/10.1002/cbin.11776 / https://www.sciencedirect.com/science/article/pii/S0946672X21000663 / https://www.mdpi.com/1648-9144/57/11/1177 / https://link.springer.com/article/10.1007/s10735-021-10006-0 / https://link.springer.com/article/10.1007/s10863-021-09895-3 / https://link.springer.com/article/10.1007/s12011-020-02322-5
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Shipping Condition	Cool pack
Available
Manufacturer - Category	Cell Apoptosis / TUNEL Assay Kits
Shipping Temperature	Ice bag
Storage Conditions	This product can be stored at -20°C for 12 months with shading light.
Assay time	3 h
Kit Type	Apoptosis/DNA Fragmentation
Test Principle	When cells undergo apoptosis, endonuclease enzymes are activated to cleave the genomic DNA between nucleosomes. When genomic DNA is cleaved, the exposed 3'-OH can be added with a biotinylated dUTP (Biotin-dUTP) due to the catalysis of Terminal Deoxynucleotidyl Transferase (TdT), followed by the binding of horseradish Peroxidase-labeled Streptavidin (Streptavidin-HRP). Thereafter, through the HRP catalysis, the apoptotic cells are marked by DAB, which can be detected by ordinary microscopy. This is the principle of TUNEL (TdT-mediated dUTP Nick-End Labeling) for detecting apoptosis.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.