Comparison

Complete Loading Control Detection Set Alpha-Tubulin, Beta-Actin, GAPDH

Item no. PRS-PSI-1838-0.1mg
Manufacturer ProSci
Amount 0.1 mg
Quantity options 0.02 mg 0.1 mg
Category
Type Detection Sets
Format Liquid
Applications WB
Specific against Human (Homo sapiens)
Citations Lambrechts A, Van Troys, M and Ampe C. The actin cytoskeleton in normal and pathological cell motility. Int. J. Biochem. Cell Biol. 2004; 36:1890-909.
Tristan C, Shahani N, Sedlak TW, et al. The diverse functions of GAPDH: views from different subcellular compartments. Cell Signal. 2011; 23:317-23.
McKean PG, Vaughan S, and Gull K. The extended tubulin family. J. Cell Sci. 2001; 114:2723-33.
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Available
Storage Conditions
Stable at 4°C for three months, store at -20°C for up to one year.
Background
Loading controls are used in immunoblots to normalize the levels of protein detected by confirming that protein loading is the same across the gel. The expression levels of the loading control should not vary between the different sample lanes. Loading controls are usually proteins that exhibit high-level, constitutive expression in the cell type or sample. This ensures constant expression levels. Thus “housekeeping genes” such as Beta-actin, Alpha-tubulin, and GAPDH are frequently chosen for use as loading controls. Actin is a ubiquitous protein and a major component of the eukaryotic cytoskeleton. Globular tubulin subunits made up of alpha- and beta-tubulin heterodimers are the building blocks of microtubules, one of three types of cytosolic fibers that comprise the cytoskeleton. GAPDH is constitutively expressed at high levels in almost all tissues and cell lines, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism.
Set Contents
Alpha-Tubulin Antibody, Catalog No. 5103

Beta-Actin Antibody (CT), Catalog No. 5005

GAPDH Antibody (CT), Catalog No. 5003
Purification
Antibodies are supplied as affinity chromatography purified IgG.
Concentration
Antibody 1 mg/mL
Buffer
PBS containing 0.02% sodium azide.
Immunogen
Rabbit polyclonal antibodies were raised against peptides corresponding to amino acid sequences from each of the corresponding proteins.
Clonality
Polyclonal
Disclaimer
This product is for research use only.
User Note
Optimal dilutions for each application to be determined by the researcher.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 0.1 mg
Available: In stock
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