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pEASY®-Blunt Simple Cloning Kit

pEASY®-Blunt Simple Cloning Kit

Item no.	TRG-CB111-02
Manufacturer	TransGen Biotech
Amount	60 rxns
Quantity options	20 rxns 60 rxns
Category	Other Assays & Kits Other Kits
Type	Kit
Specific against	other
Dry ice	Yes
Citations	1 Wu X, Yu C, Mu W, et al. The structural mechanism for transcription activation by Caulobacter crescentus GcrA[J]. Nucleic Acids Research, 2023.(IF 19.16) 2 Li Y, Du Y, Huai J, et al. The RNA helicase UAP56 and the E3 ubiquitin ligase COP1 coordinately regulate alternative splicing to repress photomorphogenesis in Arabidopsis[J]. The Plant Cell, 2022.(IF 12) 3 Wang X, Liu C, Zhang S, et al. N6-methyladenosine modification of MALAT1 promotes metastasis via reshaping nuclear speckles[J]. Developmental cell, 2021.(IF 10.09) 4 Wang H, Zhang L, Shang Y, et al. Emergence and evolution of highly pathogenic porcine epidemic diarrhea virus by natural recombination of a low pathogenic vaccine isolate and a highly pathogenic strain in the spike gene[J]. Virus evolution, 2020.(IF 5.50) 5 Li L, Fang C, Zhuang N, et al. Structural basis for transcription initiation by bacterial ECF σ factors[J]. Nature communications, 2019.(IF 11.90) 6 Zhang W, Wan H, Feng G, et al. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys[J]. Nature, 2018.(IF 41.57)
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufacturer - Category	pEASY Cloning Vectors
Storage Conditions	Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months
Product Details	pEASY®-Blunt Simple Cloning Vector eliminates the multi-cloning sites of pEASY® -Blunt Cloning Vector. It is designed for cloning and sequencing Pfu-amplified PCR products. •5 minutes fast ligation of Pfu-amplified PCR products. •Kanamycin and Ampicillin resistance genes for selection. •Easy blue/white selection. •SR primer and M13 forward primer for sequencing. •T7 promoter for in vitro transcription. •Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (> 109 cfu/μg pUC19 DNA) and fast growing. Storage Trans1-T1 Phage Resistant Chemically Competent Cell at -70°C for six months; others at -20°C for nine months Shipping Dry ice (-70℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.