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pEASY®-Blunt Zero Cloning Kit

pEASY®-Blunt Zero Cloning Kit

Item no.	TRG-CB501-01
Manufacturer	TransGen Biotech
Amount	20 rxns
Quantity options	20 rxns 60 rxns
Category	Other Assays & Kits Other Kits
Type	Kit
Specific against	other
Dry ice	Yes
Citations	1 Lin J L, Fang X, Li J X, et al. Dirigent gene editing of gossypol enantiomers for toxicity-depleted cotton seeds[J]. Nature Plants, 2023.(IF 17.35) 2 Luo Y, Liu S, Xue J, et al. High-throughput screening of spike variants uncovers the key residues that alter the affinity and antigenicity of SARS-CoV-2[J]. Cell Discovery, 2023.(IF 38.07) 3 Wen R, Wang K, Zan X. Characterization of two full-length tubuliform silk gene sequences from Neoscona theisi reveals intragenic concerted evolution and multiple copies in genome[J]. International Journal of Biological Macromolecules, 2022.(IF 8.00) 4 Luo C, Yan J, Liu W, et al. Genetic mapping and genome-wide association study identify BhYAB4 as the candidate gene regulating seed shape in wax gourd (Benincasa hispida)[J]. Frontiers in Plant Science, 2022.(IF 6.62) 5 Chen J, Hu X, Shi T, et al. Metabolite‐based genome‐wide association study enables dissection of the flavonoid decoration pathway of wheat kernels[J]. Plant Biotechnology Journal, 2020.(IF 6.84)
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufacturer - Category	pEASY Cloning Vectors
Storage Conditions	Competent Cell at -70°C for six months; others at -20°C for 9 months
Product Details	pEASY®-Blunt Zero Cloning Vector contains a suicide gene. Ligation of PCR fragment disrupts the expression of the gene. Cells that contain non-recombinant vector are killed upon plating. Therefore, blue/white selection is not required. •5 minutes fast ligation of Pfu-amplified PCR products. •High cloning efficiency. Positive clones up to 100%. •No blue/white selection needed. •Suitable for larger fragment cloning. •KAN and AMP resistance genes for selection. •M13 forward primer and M13 reverse primer for sequencing. •T3 promoter and T7 promoter for in vitro transcription. •Trans1-T1 Phage Resistant Chemically Competent Cell, high transformation efficiency (> 109 cfu/μg pUC19 DNA) and fast growing. Storage Competent Cell at -70°C for six months; others at -20°C for 9 months Shipping Dry ice (-70℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.