Anti-Mouse CD226 [Clone 10E5] – FITC - 50 µg

B, FA, FC, in vivo

2 - 8°C Wet Ice

Murine (m) CD226 (DNAM-1; PTA1) is an adhesion molecule involved in intercellularadhesion, lymphocyte signaling, cytotoxicity, and lymphokine secretion mediated by cytotoxic T-lymphocyte and natural killer (NK) cells (Uniprot Q8K4F0)1. mCD226 is also essential for NK-mediated killing of immature dendritic cells 2 and acts as an important costimulatory molecule inTh1 cell activation and effector function3.CD226 binds to PVR (CD155) via its N-terminal Ig-like domain2 and with cell surface receptorNECTIN2 (CD112)1. Based on its similarity to human CD226 (Uniprot Q15762), mCD226 likelycompetes with PVRIG for NECTIN2 binding. mCD226 is a marker for mature T cells2 and is specifically expressed on the surface ofdifferentiated Th1 cells but not Th2 or Th0 cells3. mCD226 expression is enhanced by Th1differentiation but down-regulated upon Th2 polarization. 10E5 was generated by immunizing Lewis and Lou/M rats with established murine Th1 cellclone AE7 and Th1 cell lines derived from 5B6 and DO11.10 TCR transgenic mice3. Theresulting panel was screened on polarized Th1 and Th2 T cell clones and cell lines to find anantibody that selectively recognizes only Th1 clones. 10E5 specifically recognizes CD226 on thesurface of Th1 cells. Additionally, 10E5 inhibits Ag-specific T cell expansion, reducing theexpansion of PLP 139-151-specific Th1 cells producing IFN-γ. In vivo treatment with anti-CD226 clone 10E5 inhibits T cell activation and delays the onset and reduces the severity ofTh1-mediated autoimmune disease.

0.2 mg/ml

mCD226 is expressed mainly on T cells, including on the surface ofdifferentiated Th1 cells, almost all naïve CD8+ T cells, activated CD4+ cells, about 40% ofunactivated CD4+ T cells as well as on subsets of primary CD11b+ macrophages, CD11c+dendritic cells and NK1.1 and DX5 NK cells.