Anti-Mouse F4/80 [Clone Cl:A3-1] — Purified in vivo PLATINUM™ Functional Grade - 100 mg

Primary Monoclonal Antibodies

2 - 8 °C Wet Ice

Macrophages play an important role in innate and adaptive immune system functions1. F4/80
antigen is a widely used cell-surface marker of murine macrophages1, 2 encoded by the Adgre1
locus3. F4/80 antigen is a G protein-coupled receptor with an extracellular domain containing
repeated Epidermal Growth Factor-like calcium-binding domains and as such is a member of the
EGF-TM7 protein family, which itself is a subfamily of the adhesion G protein-coupled receptors
(ADGRE) family. F4/80 is thought to have dual adhesion and signaling functions due to its
receptor domains and is known to be required for the differentiation of antigen-specific CD8+ T
regulatory cells1 . Adgre1 null mutant mice have a dysregulated autoimmune response and
regulatory T cell generation3. Additionally, unstimulated resting macrophages express F4/80 at
higher levels than activated macrophages1.

Cl:A3-1 was generated by immunizing a rat with thioglycolate-induced peritoneal macrophages
from C57BL/6 mice4. Spleen cells were fused with mouse myeloma cell line NS1. Cl:A3-1 does
not bind to macrophages via Fc receptors and is not cytotoxic. Cl:A3-1 is able to modulate
Listeria-induced cytokine signaling between macrophages and natural killer cells2.

≥ 5.0 mg/ml

F4/80 is constitutively expressed on most macrophages, except thoselocated in T cell areas and marginal zones of lymph nodes and spleen, as well as on somedendritic cells. Blood monocytes also express F4/80, but at low levels relative to maturemacrophages.