mAbMods™ Anti-Mouse CD223 (LAG-3) Antibody - Fc muted™ (Clone C9B7W (D256A))

Recombinant Antibodies>Chimeric Antibody|Primary Monoclonal Antibodies>Immune Checkpoints|Purified in vivo Functional Grade|Primary Monoclonal Antibodies>Surfacetag Mouse CD Markers

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This anti-Lag-3 antibody is a recombinant chimeric version of the original clone C9B7W antibody. The variable domain sequences are identical to the original clone C9B7W; however, the constant region sequences have been switched from rat IgG1 to mouse IgG1. In this Fc Muted™ format, mutations (D265A) have been introduced that reduce or eliminate Fc-mediated Antibody-Dependent Cellular Phagocytosis (ADCP) and Antibody-Dependent Cellular Cytotoxicity (ADCC).
Lymphocyte activation gene (LAG)-3 is a CD4-related cell surface molecule that binds to MHC class II molecules and acts as a negative regulator of T cell function by limiting the expansion of activated T cells and T cell homeostasis 1, 2, 3. LAG-3 inhibits CD4-dependent T cell function via its cytoplasmic domain and a conserved “KIEELE” motif is essential for interaction with downstream signaling molecules 2.
Murine LAG-3 RNA is strongly expressed in the thymus, spleen, and areas of the brain outside the cerebellum as well as in day 7 postnatal brain at the base of the cerebellum, developing white matter and choroid plexus. However, LAG-3 protein is detected on the cell surface of a very small percentage of T and natural killer cells in naïve mice, meaning RNA abundance is not indicative of protein levels for this gene.
The original clone C9B7W was generated by immunizing Lewis rats with an mLAG-3:Ig fusion protein consisting of the entire extracellular domain of LAG-3 attached to the murine IgG1 heavy chain 1. C9B7W recognizes an epitope in the D2 domain of LAG-3 and does not block binding of MHC class II:γ2aFc-Alexa633 to murine LAG-3+ T cell hybridomas. However, C9B7W does inhibit antigen-induced IL-2 production.

This recombinant monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added. Due to inherent biochemical properties of antibodies, certain products may be prone to precipitation over time. Precipitation may be removed by aseptic centrifugation and/or filtration.