Anti-Mouse TIM-2 - Purified <i>in vivo </i>PLATINUM™ Functional Grade - 25 mg

Agonist, B, FA, FC, IP, WB

2 - 8°C Wet Ice

The T cell/transmembrane, immunoglobulin, and mucin (TIM) family plays a critical role inimmune response, transplant tolerance, autoimmunity, and allergy and asthma regulation1. TheTIM family consists of eight members, including TIM-2 which is only found in rodent genomes.TIM-2 is likely a gene duplication of TIM-1. TIM-2 is a type I cell surface glycoprotein that hasan N-terminal immunoglobulin-like (Ig) domain, a mucin domain with O- and N-linkedglycosylations, a single transmembrane domain, and a cytoplasmic region with tyrosinephosphorylation motif. Unlike other TIM family proteins, TIM-2 does not bind PtdSer. TIM-2 isa receptor for H-ferritin. TIM-2 functions as a negative regulator of T cell activation and Th2responses.RMT2-25 was generated by immunizing Sprague Dawley rats with a TIM-2-Ig chimera proteinconsisting of murine TIM-2-Ig (extracellular domain residues 1-230) and the Fc portion ofmouse IgG2a2 . LN cells were fused with P3U1 myeloma cells. The RMT2-25 hybridoma wasselected using flow cytometry, based on reactivity to mouse TIM-2 transfected cells, but notparental cells. RMT2-25 inhibits the binding of H-ferritin to TIM-2. RMT2-25 also significantlyenhances B cell proliferation and activation in vitro by agonistic stimulation of TIM-2.

≥ 5.0 mg/ml

TIM-2 is expressed by activated T cells, particularly after differentiationunder Th2 conditions. TIM-2 is not expressed by T cells differentiated under Th1 conditions.TIM-2 is also expressed by splenic and germinal B cells. Other cells expressing TIM-2 includeepithelial cells in the liver and kidney as well as oligodendrocytes.