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293T Lysate

293T Lysate

Item no.	NKM-ATGL0002-100ug
Manufacturer	NKMAX
Amount	100 ug
Quantity options	100 ug 200 ug 500 ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Format	Liquid
Applications	WB
Specific against	Human (Homo sapiens)
Citations	Mizuno R, et al. Expression and localization of anti-Müllerian hormone and its receptors in bovine corpus luteum. (Theriogenology. 2024)
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Similar products	293T, ATGL0002-10ug, ATGL0002-20ug, ATGL0002-50ug, ATGL0002-100ug, ATGL0002-250ug, ATGL0002-500ug, ATGL0002-1mg, ATGL0002-10, ATGL0002-20, ATGL0002-50, ATGL0002-100, ATGL0002-250, ATGL0002-500, ATGL0002-1
Available
Manufacturer - Type	Lysates
Manufacturer - Category	Cell Lysate
Storage Conditions	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description	293T cells are a human embryonic kidney (HEK) cell line that expresses the large T antigen. The large T antigen is commonly used for transfection assays and is required for transient transfection of plasmids with an SV40 origin of replication. 293T cells are able to grow well in reduced serum medium or in medium lacking calcium ions. In addition, 293T cells tend to grow at a faster rate and to a higher cell density than the parent 293 cell line, resulting in increased amounts of endogenously expressed protein.
Concentration	2mg/ml (determined by BCA assay)
Formulation	Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species	Human
Gene Category	Lysates
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein	Human
Manufacturer - Search Terms	293T, ATGL0002-10ug, ATGL0002-20ug, ATGL0002-50ug, ATGL0002-100ug, ATGL0002-250ug, ATGL0002-500ug, ATGL0002-1mg, ATGL0002-10, ATGL0002-20, ATGL0002-50, ATGL0002-100, ATGL0002-250, ATGL0002-500, ATGL0002-1
WB Comment	All lanes : 293T Whole Cell Lysate (40ug) Lane 1. : Anti-GAPDH antibody (ATGA0181) Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 100 ug

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Delivery expected until 10/30/2025

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