Comparison

Jurkat Lysate

Item no. NKM-ATGL0003-200ug
Manufacturer NKMAX
Amount 200 ug
Quantity options 100 ug 200 ug 500 ug
Category
Type Cell Lysate
Format Liquid
Applications WB
Specific against Human (Homo sapiens)
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41105330
Similar products Jurkat, ATGL0003-10ug, ATGL0003-20ug, ATGL0003-50ug, ATGL0003-100ug, ATGL0003-250ug, ATGL0003-500ug, ATGL0003-1mg, ATGL0003-10, ATGL0003-20, ATGL0003-50, ATGL0003-100, ATGL0003-250, ATGL0003-500, ATGL0003-1
Available
Manufacturer - Type
Lysates
Manufacturer - Category
Cell Lysate
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description
Jurkat cells are an immortalized line of human T lymphocyte cells take from the peripheral blood of a 14-year-old boy with T cell leukemia, in 1970s. Jurkat cells are used to study acute T cell leukemia, T cell signaling, and the expression of various chemokine receptors susceptible to viral entry, particularly HIV. Jurkat cells are also useful in science because of their ability to produce interleukin 2. Their primary use, however, is to determine the mechanism of differential susceptibility of cancers to drugs and radiation.
Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species
Human
Gene Category
Lysates
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein
Human
Manufacturer - Search Terms
Jurkat, ATGL0003-10ug, ATGL0003-20ug, ATGL0003-50ug, ATGL0003-100ug, ATGL0003-250ug, ATGL0003-500ug, ATGL0003-1mg, ATGL0003-10, ATGL0003-20, ATGL0003-50, ATGL0003-100, ATGL0003-250, ATGL0003-500, ATGL0003-1
WB Comment
All lanes : Jurkat Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ug
Available: In stock
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