Comparison

A549 Lysate

Item no. NKM-ATGL0008-200ug
Manufacturer NKMAX
Amount 200 ug
Quantity options 100 ug 200 ug 500 ug
Category
Type Cell Lysate
Format Liquid
Applications WB
Specific against Human (Homo sapiens)
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41105330
Similar products A549, ATGL0008-10ug, ATGL0008-20ug, ATGL0008-50ug, ATGL0008-100ug, ATGL0008-250ug, ATGL0008-500ug, ATGL0008-1mg, ATGL0008-10, ATGL0008-20, ATGL0008-50, ATGL0008-100, ATGL0008-250, ATGL0008-500, ATGL0008-1
Available
Manufacturer - Type
Lysates
Manufacturer - Category
Cell Lysate
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description
A549 cells are adenocarcinomic human alveolar basal epithelial cells. The A549 cell line was initiated from the removal and culturing of cancerous lung tissue in the explanted tumor from 58-year-old male, in 1972. A549 cells is able to synthesize lecithin and contain high level of unsaturated fatty acids, which are important to maintain the membrane phospholipids in cells. A549 cell line are widely used as an in vitro model for a type II pulmonary epithelial cell model for drug metabolism and as a transfection host.
Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species
Human
Gene Category
Lysates
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein
Human
Manufacturer - Search Terms
A549, ATGL0008-10ug, ATGL0008-20ug, ATGL0008-50ug, ATGL0008-100ug, ATGL0008-250ug, ATGL0008-500ug, ATGL0008-1mg, ATGL0008-10, ATGL0008-20, ATGL0008-50, ATGL0008-100, ATGL0008-250, ATGL0008-500, ATGL0008-1
WB Comment
All lanes : A549 Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 200 ug
Available: In stock
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