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MCF-7 Lysate

MCF-7 Lysate

Item no.	NKM-ATGL0009-200ug
Manufacturer	NKMAX
Amount	200 ug
Quantity options	100 ug 200 ug 500 ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Format	Liquid
Applications	WB
Specific against	Human (Homo sapiens)
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Similar products	MCF7, ATGL0009-10ug, ATGL0009-20ug, ATGL0009-50ug, ATGL0009-100ug, ATGL0009-250ug, ATGL0009-500ug, ATGL0009-1mg, ATGL0009-10, ATGL0009-20, ATGL0009-50, ATGL0009-100, ATGL0009-250, ATGL0009-500, ATGL0009-1
Available
Manufacturer - Type	Lysates
Manufacturer - Category	Cell Lysate
Storage Conditions	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description	MCF-7 cells are breast cancer cell line isolated in 1970 from a 69 year old woman, in 1970s. MCF-7 cells are a well characterized estrogen receptor (ER) positive control cell line (cells are positive for cytoplasmic estrogen receptors) and therefore are a useful in vitro model of breast cancer to study the role of estrogen in breast cancer. Cells are also positive for cytokeratin and negative for desmin, endothelin, GFAP, neurofilament, vimentin.
Concentration	2mg/ml (determined by BCA assay)
Formulation	Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species	Human
Gene Category	Lysates
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein	Human
Manufacturer - Search Terms	MCF7, ATGL0009-10ug, ATGL0009-20ug, ATGL0009-50ug, ATGL0009-100ug, ATGL0009-250ug, ATGL0009-500ug, ATGL0009-1mg, ATGL0009-10, ATGL0009-20, ATGL0009-50, ATGL0009-100, ATGL0009-250, ATGL0009-500, ATGL0009-1
WB Comment	All lanes : MCF-7 Whole Cell Lysate (40ug) Lane 1. : Anti-GAPDH antibody (ATGA0181) Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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NKM-ATGL0009-200ug-datasheet.pdf

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Amount: 200 ug

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Delivery expected until 10/30/2025

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