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NIH/3T3 Lysate

NIH/3T3 Lysate

Item no.	NKM-ATGL0017-500ug
Manufacturer	NKMAX
Amount	500 ug
Quantity options	100 ug 200 ug 500 ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Format	Liquid
Applications	WB
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Similar products	NIHT3, ATGL0017-10ug, ATGL0017-20ug, ATGL0017-50ug, ATGL0017-100ug, ATGL0017-250ug, ATGL0017-500ug, ATGL0017-1mg, ATGL0017-10, ATGL0017-20, ATGL0017-50, ATGL0017-100, ATGL0017-250, ATGL0017-500, ATGL0017-1
Available
Manufacturer - Type	Lysates
Manufacturer - Category	Cell Lysate
Storage Conditions	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description	NIH/3T3 cell line was established from NIH Swiss mouse embryo cultures in the same manner as the original random bred 3T3 and the inbred BALB/c 3T3. These cells are highly contact inhibited and are sensitive to sarcoma virus focus formation and leukaemia virus propagation. Cells have now lost their contact inhibition.
Concentration	2mg/ml (determined by BCA assay)
Formulation	Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species	Mouse
Gene Category	Lysates
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein	Mouse
Manufacturer - Search Terms	NIHT3, ATGL0017-10ug, ATGL0017-20ug, ATGL0017-50ug, ATGL0017-100ug, ATGL0017-250ug, ATGL0017-500ug, ATGL0017-1mg, ATGL0017-10, ATGL0017-20, ATGL0017-50, ATGL0017-100, ATGL0017-250, ATGL0017-500, ATGL0017-1
WB Comment	All lanes : NIH/3T3 Whole Cell Lysate (40ug) Lane 1. : Anti-GAPDH antibody (ATGA0181) Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 500 ug

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Delivery expected until 10/30/2025

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