Comparison

WiDr Lysate

Item no. NKM-ATGL0020-500ug
Manufacturer NKMAX
Amount 500 ug
Quantity options 100 ug 200 ug 500 ug
Category
Type Cell Lysate
Format Liquid
Applications WB
Specific against Human (Homo sapiens)
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41105330
Similar products WiDr, ATGL0020-10ug, ATGL0020-20ug, ATGL0020-50ug, ATGL0020-100ug, ATGL0020-250ug, ATGL0020-500ug, ATGL0020-1mg, ATGL0020-10, ATGL0020-20, ATGL0020-50, ATGL0020-100, ATGL0020-250, ATGL0020-500, ATGL0020-1
Available
Manufacturer - Type
Lysates
Manufacturer - Category
Cell Lysate
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description
WiDr cells are established from a 78 year old female with colon adenocarcinoma. The cells are negative for colon antigen 3 expression and positive for keratin by immunoperoxidase staining. WiDr cells expressed p53 antigen. Growth of WiDR cells is inhibited by tumor necrosis factor alpha (TNF-alpha). Inhibitors of dihydrofolate reductase are highly cytotoxic to WiDR cells.
Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species
Human
Gene Category
Lysates
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein
Human
Manufacturer - Search Terms
WiDr, ATGL0020-10ug, ATGL0020-20ug, ATGL0020-50ug, ATGL0020-100ug, ATGL0020-250ug, ATGL0020-500ug, ATGL0020-1mg, ATGL0020-10, ATGL0020-20, ATGL0020-50, ATGL0020-100, ATGL0020-250, ATGL0020-500, ATGL0020-1
WB Comment
All lanes : WiDr Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
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