« Back
Home /
B16-F10 Lysate

B16-F10 Lysate

Item no.	NKM-ATGL0025-500ug
Manufacturer	NKMAX
Amount	500 ug
Quantity options	100 ug 200 ug 500 ug
Category	Western Blot Cell Lines & Cell Culture Cell Lysates
Type	Cell Lysate
Format	Liquid
Applications	WB
Specific against	Mouse (Murine, Mus musculus)
ECLASS 10.1	32160890
ECLASS 11.0	32160890
UNSPSC	41105330
Similar products	B16-F10, ATGL0025-10ug, ATGL0025-20ug, ATGL0025-50ug, ATGL0025-100ug, ATGL0025-250ug, ATGL0025-500ug, ATGL0025-1mg, ATGL0025-10, ATGL0025-20, ATGL0025-50, ATGL0025-100, ATGL0025-250, ATGL0025-500, ATGL0025-1
Available
Manufacturer - Type	Lysates
Manufacturer - Category	Cell Lysate
Storage Conditions	Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description	B16-F10 cells are derived from mouse skim melanoma. B16 melanoma cell line and its sublines are well-established models for the study of experimental cancer therapies. Melanomas express different tumor-associated Ags, which are potential targets for novel designs of therapeutic cancer vaccines. Many immunotherapeutic protocols have been tested using the B16 cell line. Growth of B16 cells can be controlled in vivo by different classes of specific or nonspecific immune effector cells.
Concentration	2mg/ml (determined by BCA assay)
Formulation	Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species	Mouse
Gene Category	Lysates
Preparation before usage	Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC	Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein	Mouse
Manufacturer - Search Terms	B16-F10, ATGL0025-10ug, ATGL0025-20ug, ATGL0025-50ug, ATGL0025-100ug, ATGL0025-250ug, ATGL0025-500ug, ATGL0025-1mg, ATGL0025-10, ATGL0025-20, ATGL0025-50, ATGL0025-100, ATGL0025-250, ATGL0025-500, ATGL0025-1
WB Comment	All lanes : B16-F10 Whole Cell Lysate (40ug) Lane 1. : Anti-GAPDH antibody (ATGA0181) Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Request Data Sheet

NKM-ATGL0025-500ug-datasheet.pdf

Request safety datasheet

Simple Order Order Subscription

Amount: 500 ug

available

Delivery expected until 10/30/2025

Express Delivery: Save 3 days

Compare

Add to wishlist

Get an offer