Comparison

L-929 Lysate

Item no. NKM-ATGL0031-100ug
Manufacturer NKMAX
Amount 100 ug
Quantity options 100 ug 200 ug 500 ug
Category
Type Cell Lysate
Format Liquid
Applications WB
Specific against Mouse (Murine, Mus musculus)
ECLASS 10.1 32160890
ECLASS 11.0 32160890
UNSPSC 41105330
Similar products L929, ATGL0031-10ug, ATGL0031-20ug, ATGL0031-50ug, ATGL0031-100ug, ATGL0031-250ug, ATGL0031-500ug, ATGL0031-1mg, ATGL0031-10, ATGL0031-20, ATGL0031-50, ATGL0031-100, ATGL0031-250, ATGL0031-500, ATGL0031-1
Available
Manufacturer - Type
Lysates
Manufacturer - Category
Cell Lysate
Storage Conditions
Aliquot the lysate into single tube and then storing them -80C or below. Avoid repeated freezing and thawing cycles.
Description
L-929 mouse fibroblast cells line established by W. R Earle in 1940. This strain was derived from normal subcutaneous areolar and adipose tissue of a 100 day old male C3H/An mouse. Cells are APRT+ and HPRT+. L929 cells are used in studies of TNF-alpha, TNF-beta bioassays and cytokine assays.
Concentration
2mg/ml (determined by BCA assay)
Formulation
Liquid in. SDS-PAGE Sample Buffer (0.12M Tris-HCl buffer pH6.8, 0.4% SDS, 5% glycerol, 1% beta-mercaptoethanol, 0.02% Bromophenol blue)
Antigen Species
Mouse
Gene Category
Lysates
Preparation before usage
Heat lysate to 95C for 5 minutes and rapidly cooling before use.
Lysate QC
Western Blot analyzed by both GAPDH and Beta tubulin antibody
Species of Protein
Mouse
Manufacturer - Search Terms
L929, ATGL0031-10ug, ATGL0031-20ug, ATGL0031-50ug, ATGL0031-100ug, ATGL0031-250ug, ATGL0031-500ug, ATGL0031-1mg, ATGL0031-10, ATGL0031-20, ATGL0031-50, ATGL0031-100, ATGL0031-250, ATGL0031-500, ATGL0031-1
WB Comment
All lanes : L-929 Whole Cell Lysate (40ug)

Lane 1. : Anti-GAPDH antibody (ATGA0181)
Lane 2. : Anti-Beta tubulin antibody (ATGA0196)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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