Comparison

PRELP Protein Lysate

Item no. OCOA03816
Manufacturer AVIVA Systems Biology
Amount 20 ug
Category
Type LYSATE
Format Lyophilized
Specific against Human (Homo sapiens)
Host HEK293
Conjugate/Tag Myc
Dry ice Yes
ECLASS 10.1 32190102
ECLASS 11.0 32190102
UNSPSC 41105330
Alias 55 kDa leucine-rich repeat protein of articular cartilage;MST161;MSTP161;prolargin;prolargin proteoglycan;proline-arginine-rich end leucine-rich repeat protein;SLRR2A.
Shipping Condition Dry ice
Available
Manufacturer - Type
Protein Lysate
Manufacturer - Category
Root Catalog/Products/Other/Lysate
Manufacturer - Conjugate / Tag
C-MYC/DDK
Shipping Temperature
Dry Ice
Molecular Weight
43.8 kDa
Gene symbol
PRELP
Gene Fullname
proline and arginine rich end leucine rich repeat protein
Product format
Lyophilized
Reconstitution and storage
The lysate can be shipped at ambient temperature. Upon receiving, store the sample at -20C. Lysate samples are stable for 12 months from date of receipt when stored at -20C. Avoid repeated freeze-thaw cycles after reconstitution. Lysate samples can be reconstituted with SDS Sample Buffer. Prior to SDS-PAGE fractionation, boil the lysate for 5 minutes
Description of target
Homo sapiens proline and arginine rich end leucine rich repeat protein (PRELP), transcript variant 2, mRNA, Prolargin, prolargin precursor [Homo sapiens]
Nucleotide accession_num
NM_201348
Protein accession_num
https://www.ncbi.nlm.nih.gov/protein/NP_958505
Protein name
NM_201348, NP_958505, P51888
Preparation
HEK293T cells in 10-cm dishes were transiently transfected with MegaTran Transfection Reagent and 5ug TrueORF cDNA plasmid. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix (Sigma), 1mM PMSF and 1mM Na3VO4), and then centrifuged to clarify the lysate. Protein concentration was measured by BCA kit (Thermo Scientific Inc.). To facilitate transportation and protein, the products are supplied as lyophilized proteins.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 20 ug
Available: In stock
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