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Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B)

Biotinylated Cynomolgus MADCAM1 Protein (MCM-CM401B)

Item no.	MCM-CM401B-100UG
Manufacturer	KactusBio
Amount	100 ug
Quantity options	100 ug 500 ug
Category	Cell Biology Cell Movement Cell Adhesio
Format	Lyophilized
Specific against	Monkey (Cynomolgus, Simian)
Host	HEK293
Conjugate/Tag	HIS, Avi
Purity	> 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Sequence	Gln22-Gln295
NCBI	XP_045235080.2
Alias	MAdCAM-1,MADCAM1,MACAM1
Shipping Condition	Room temperature
Available
Manufacturer - Type	Recombinant Protein
Manufacturer - Targets	MADCAM1
Manufacturer - Conjugate / Tag	C-His-Avi
Shipping Temperature	Shipped at ambient temperature.
Storage Conditions	-20 to -80°C for 12 months as supplied from date of receipt.；-80°C for 3 months after reconstitution.；Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight	The protein has a predicted MW of 31.95 kDa. Due to glycosylation, the protein migrates to 60-70 kDa based on Bis-Tris PAGE result.
Description	Recombinant Biotinylated Cynomolgus MADCAM1 Protein is expressed from HEK293 with His tag and Avi tag at the C-terminus.It contains Gln22-Gln295.
Background	Mucosal addressin cell adhesion molecule-1 (MAdCAM-1) contributes to the recruitment of donor T cells into the mucosal tissues of the recipient after allogeneic hematopoietic stem cell transplantation (aHSCT). The aim of our study was to determine whether selected single nucleotide polymorphisms (SNPs) of the MADCAM1 gene are associated with development of serious complications after aHSCT.
Endotoxin	Less than 1 EU per μg by the LAL method.
Formulation	Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
Reconstitution	Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated	yes

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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MCM-CM401B-100UG-safety-datasheet.pdf