Comparison

Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein (MHC-HE011B)

Item no. MHC-HE011B-500UG
Manufacturer KactusBio
Amount 500 ug
Quantity options 100 ug 500 ug
Category
Type Proteins Recombinant
Format Lyophilized
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag HIS, Avi
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Sequence Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and HMTEVVRHC peptide
NCBI HMTEVVRHC
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias MHC,HLA-A,P53,TP53,Antigen NY-CO-13,BCC7,FLJ92943,LFS1,TRP53
Shipping Condition Room temperature
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Targets
HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC)
Manufacturer - Conjugate / Tag
C-His-Avi
Shipping Temperature
Shipped at ambient temperature.
Storage Conditions
-20 to -80°C for 12 months as supplied from date of receipt.;-80°C for 3 months after reconstitution.;Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight
The protein has a predicted MW of 35.6 kDa (HLA-A*02:01) and 11.9 kDa (B2M) same as Bis-Tris PAGE result.
Description
Recombinant Biotinylated Human HLA-A*02:01&B2M&P53 R175H (HMTEVVRHC) Monomer Protein is expressed from E.coli with His tag and Avi tag at the C-terminus.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and HMTEVVRHC peptide.
Background
p53 is a tumor suppressor protein. Under stressful conditions, p53 tightly regulates cell growth by promoting apoptosis and DNA repair. When p53 becomes mutated, it loses its function, resulting in abnormal cell proliferation and tumor progression. Depending on the p53 mutation, it has been shown to form aggregates leading to negative gain of function of the protein.p53 mutant associated aggregation has been observed in several cancer tissues and has been shown to promote tumor growth.
Endotoxin
Less than 1 EU per μg by the LAL method.
Formulation
Lyophilized from 0.22 μm filtered solution in 20mM Tris, 200mM NaCl (pH 8.0). Normally 8% mannitol is added as protectant before lyophilization.
Reconstitution
Dissolve the lyophilized protein in distilled water. It is recommended to resuspend at 0.5 mg/mL if the lyophilized powder is 100 μg or less, at 1mg/ml for 500μg or 1mg lyophilized powder. Do not mix by vortex or vigorous shaking.
Biotinylated
yes

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 500 ug
Available: In stock
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