Comparison

Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer Protein (MHC-HE013T)

Item no. MHC-HE013T-100UG
Manufacturer KactusBio
Amount 100 ug
Quantity options 100 ug 500 ug
Category
Type Proteins Recombinant
Format Liquid
Specific against Human (Homo sapiens)
Host E.coli
Conjugate/Tag HIS, Avi
Purity > 95% as determined by Bis-Tris PAGE;> 95% as determined by HPLC
Dry ice Yes
Sequence Gly25-Thr305(HLA-A*02:01), Ile21-Met119(B2M) and NLVPMVATV peptide
NCBI NLVPMVATV
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CMVpp65,NLVPMVATV
Shipping Condition Dry ice
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Targets
HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV)
Manufacturer - Conjugate / Tag
C-His-Avi
Shipping Temperature
Shipped with dry ice.
Storage Conditions
Valid for 12 months from date of receipt when stored at -80°C.; Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
Molecular Weight
The protein has a predicted MW of 258 kDa. The protein migrates to 120-180 kDa (HLA-A*02:01&streptavidin tetramer) and 11.9 kDa (B2M) based on Bis-Tris PAGE result under Non reducing (N) condition.
Description
Recombinant Human HLA-A*02:01&B2M&CMVpp65 (NLVPMVATV) Tetramer Protein is expressed from E.coli with His tag and Avi tag at the C-Terminus, tetramer is assembled by biotinylated monomer and streptavidin.It contains Gly25-Thr305 (HLA-A*02:01), Ile21-Met119 (B2M) and NLVPMVATV peptide.
Background
Human cytomegalovirus (CMV), a β-herpes virus with a double-stranded DNA, infects a wide variety of cells and establishes latency in the host. CMVpp65, a tegument protein of the herpes virus CMV, is the main viral antigen found in peripheral blood mononuclear cells (PBMCs) after viral infection and may activate cell-mediated immunity, accounting for 70-90% of the cytotoxic CD8+ T cells' (CTLs) response to CMV. Among the pp65-derived CTL epitope peptides, the 9-mer peptide 495NLVPMVATV503 (CMVpp65 495-503 peptide) is the most immunogenic T cell epitope predominantly displayed on HLA-A*02:01, the most common MHC-I allele in the population.
Endotoxin
Less than 1 EU per μg by the LAL method.
Formulation
Supplied as 0.22 μm filtered solution in PBS (pH 7.4).
Biotinylated
no

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 100 ug
Available: In stock
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