Ionomycin

56092-81-0

C41H72O9

Ethanol : >= 10 mg/mL (14.10 mM)

Membrane Transporter/Ion Channel; Neuronal Signaling

Ionomycin (SQ23377) is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus. IC50 & Target: Calcium channel^[1]. In Vitro: Ionomycin (SQ23377) is a Calcium ionophore and an antibiotic produced by Streptomyces conglobatus ATCC 31005^[1]. It is observed that LCLC 103H cells overexpressing the catalytic subunit of u-calpain rapidly underwent cell death after treatment with ionomycin. The first signs of Ionomycin (SQ23377)-induced cell death are detected 3 h after addition of the ionophore. Addition of 2 uM Ionomycin (SQ23377) to LCLC 103H cells causes an instantaneous increase in intracellular Ca²⁺ concentration from 50 to 180 nM. Remarkably, calcium concentrations are raised transiently to 0.8-1.5 uM. DNA and protein analysis in Ionomycin (SQ23377)-treated cultures revealed DNA fragmentation and PARP cleavage to an 85-kDa fragment typical of caspase-mediated apoptosis. Three hours after addition of ionomycin, the percentage of early apoptotic cells approximately doubles. Necrosis could be detected in ca.1-5% of the Ionomycin (SQ23377) treated cells as supported by simultaneously positive fluorescein labeling and propidium iodide uptake. Caspase activation in whole cells was followed by monitoring the increase in activity against Ac-DEVD-amc following Ionomycin (SQ23377) treatment^[2].