GPR84 antagonist 8

1445846-30-9

C23H23N3O5

DMSO : 5 mg/mL (11.86 mM; Need ultrasonic)

GPCR/G Protein

GPR84 antagonist 8 is a selective GPR84 antagonist. IC50 & Target: GPR84^[1] In Vitro: GPR84 is a member of the metabolic G protein-coupled receptor family, and its expression has been described predominantly in immune cells. To test the hypothesis that blocking the activation of GPR84 can be a potential anti-inflammatory strategy in different inflammatory diseases, GPR84 antagonist 8 is used. The potency and specificity of GPR84 antagonist 8 is assessed tusing GPR84-CHO cells in the cAMP assay. GPR84 antagonist 8 effectively inhibits the action of 6-OAU in decreasing cAMP production in GPR84-CHO cells. To test GPR84 antagonist 8�s inhibition of the pro-inflammatory effects of GPR84 activation in macrophages, LPS pre-treated BMDMs are incubated with 10?uM GPR84 antagonist 8 for 30?min before adding 1?uM 6-OAU. Protein analysis by Western Blot shows that the GPR84 antagonist 8 partially blocks the phosphorylation of AKT and ERK induced by 6-OAU^[1].