7alpha,25-Dihydroxycholesterol

64907-22-8

418.65

DMSO : 1 mg/mL (2.39 mM; Need ultrasonic); Ethanol : < 1 mg/mL (insoluble)

GPCR/G Protein; Metabolic Enzyme/Protease

7alpha, 25-dihydroxycholesterol (7alpha, 25-OHC) is a potent and selective agonist and endogenous ligand of the orphan GPCR receptor EBI2 (GPR183). 7alpha, 25-dihydroxycholesterol is highly potent at activating EBI2 (EC50=140 pM; Kd=450 pM). 7alpha, 25-dihydroxycholesterol can serve as a chemokine directing migration of B cells, T cells and dendritic cells.
In Vitro: In vitro, 7alpha, 25-dihydroxycholesterol (7alpha, 25-OHC) stimulates the migration of EBI2-expressing mouse B and T cells with half-maximum effective concentration values around 500 pM, but had no effect on EBI2-deficient cells.
In Vivo: EBI2-deficient B cells or normal B cells desensitized by 7alpha, 25-Dihydroxycholesterol (1 uM; 1.5 hours) pre-treatment shows reduced homing to follicular areas of the spleen.