Pseudocoptisine (chloride)

30044-78-1

355.77

10 mM in DMSO

Neuronal Signaling

Pseudocoptisine (Isocoptisine) chloride is a quaternary alkaloid with benzylisoquinoline skeleton, was isolated from Corydalis Tuber. Pseudocoptisine chloride inhibits acetylcholinesterase (AChE) activity with an IC50 of 12.8 uM. Anti-inflammatory and anti-amnestic effects.
In Vitro: Pseudocoptisine (0, 60, 90 uM; 1 hour) dose-dependently inhibited LPS-induced NO production in RAW264.7 cells.

Pseudocoptisine (30-90 uM; 1 hour; RAW264.7 cells) significantly reduces the LPS-induced TNF-alpha and IL-6 production and their mRNA expressions.

Pseudocoptisine acetate reduces levels of the pro-inflammatory mediators, such as, iNOS, COX-2, TNF-alpha, and IL-6 through the inhibition of NF-kappaB activation via the suppression of ERK and p38 phosphorylation in RAW 264.7 cells.
In Vivo: The anti-amnesic activities of Pseudocoptisine in mice on the learning and memory impairments induced by scopolamine (1.0 mg/kg, i.p.) are examined. Pseudocoptisine (2.0 mg/kg, p.o.) significantly reverses cognitive impairments in mice by passive avoidance test.