IBMX

28822-58-4

C10H14N4O2

DMSO : 100 mg/mL (449.96 mM; Need ultrasonic)

Metabolic Enzyme/Protease

IBMX is a broad-spectrum phosphodiesterase (PDE) inhibitor, with IC₅₀s of 6.5, 26.3 and 31.7 uM for PDE3, PDE4 and PDE5, respectively. IC50 & Target: IC50: 6.5+/-1.2 uM(PDE3), 26.3+/-3.9 uM (PDE4), 31.7+/-5.3 uM (PDE5)^[1] In Vitro: At 100 uM, KMUP-1 (a xanthine derivative) and IBMX are the most effective at inducing tracheal relaxation; the magnitude of the relaxation responses induced by KMUP-1 and IBMX are not significantly different^[1]. IBMX (100 uM) activates renal outer medullary K⁺ (ROMK) channels (n=6, P<0.05) and prevents further channel activation by ANG II (n=6, P=NS) or cGMP. Of note is that pretreatment of cortical collecting duct (CCDs) isolated from high-K⁺ (HK)-fed rats with IBMX (100 uM) for 20 min leads to a significant increase in tubular cAMP content to 1.43+/-0.35 pg/mm tubule length (n=14) compare with that measured in vehicle-treated controls (0.61+/-0.13 pg/mm tubule length, n=12, P<0.05)^[2]. In Vivo: IBMX, a non-selective PDE inhibitor significantly decreases the liver glycogen storage (mg/g, IBMX 22+/-1.5 P<0.001). IBMX potentiates insulin release and in hepatocytes and adipocytes, they increase glycogenolysis and lipolysis. In comparison with the control group, IBMX and mc5 significantly increase plasma glucose (blood glucose, mg/dl, control=141+/-3, IBMX=210+/-17 P<0.001 and mc5=191+/-13 P<0.01) while other test compounds (mc1, mc6, MCPIP and milrinone) do not produce significant effect (control=141+/-3, mc1 160+/-7, mc6 175+/-9, MCPIP 179+/-8 and milrinone 116+/-2 P>0.05) also mc2 does not change plasma glucose (control=141+/-3 and mc2=145+/-5). IBMX has the highest efficacy on increasing plasma glucose^[3]. Treatments with IBMX and Apocynin significantly decrease cold-induced elevation of right ventricular (RV) systolic pressure (23.5+/-1.8 and 24.2+/-0.6 mmHg, respectively) although they do not decrease RV pressure to the warm control levels. IBMX or Apocynin significantly reduces medial layer thickness (19.0+/-0.9, and 16.9+/-0.8 um, respectively) and increases lumen diameter (62.7+/-4.2, and 59.5+/-4.3 um, respectively) of small PAs in cold-exposed rats^[4].