3-Methyladenine

5142-23-4

C6H7N5

H2O : 4 mg/mL (26.82 mM; Need ultrasonic); DMSO : 22.14 mg/mL (148.44 mM; Need ultrasonic)

PI3K/Akt/mTOR; Autophagy; Autophagy; Metabolic Enzyme/Protease

3-Methyladenine is a PI3K inhibitor. 3-Methyladenine is a widely used inhibitor of autophagy via its inhibitory effect on class III PI3K. IC50 & Target: IC50: 25 uM (Vps34), 60 uM (PI3Kgamma)^[1] In Vitro: 3-Methyladenine (0-10 mM; 0-48 hours) induces caspase-dependent cell death in HeLa cells in a time-and dose-dependent manner^[2].
3-Methyladenine (5 mM; 24 hours) suppresses autophagy in HeLa cells under both glucose-free conditions and normal conditions^[2].
3-Methyladenine (5 mM; 0-48 hours) suppresses conversion of LC3-I to LC3-II (autophagy markers) between 12hours and 48 hours, confirms the inhibitory effects on autophagy^[2].
3-Methyladenine induces cell death is independent of autophagy inhibition^[2].
3-Methyladenine significantly shortens the duration of nocodazole-induced-prometaphase arrest^[2].
In Vivo: 3-Methyladenine (1.5?mg/100?g; intraperitoneal injection; 3-24?hours) treatment alleviates sodium taurocholate-induced severe acute pancreatitis (SAP) in rats at both 12 hours and 24?hours^[3].
3-Methyladenine inhibits autophagy of pancreatic acinar cells in sodium taurocholate-tnduced SAP^[3].
3-Methyladenine also shows inhibitory effects on PI3K/Akt signaling pathway and NF-kappaB signaling pathway in sodium taurocholate-induced SAP^[3].