Ro 67-7476

298690-60-5

319.39

DMSO : >= 40 mg/mL (125.24 mM)

GPCR/G Protein; Neuronal Signaling

Ro 67-7476 is a potent positive allosteric modulator of mGluR₁ and potentiates glutamate-induced calcium release in HEK293 cells expressing rat mGluR1a with an EC₅₀ of 60.1 nM^[1][2]. Ro 67-7476 is a potent P-ERK1/2 agonist and activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC₅₀=163.3 nM)^[3]. In Vitro: In the Purkinje cells of rat cerebellar slices, Ro 67-7476 increases the amplitude of mGluR1 excitatory postsynaptic potentials (EPSCs) evoked by 2, 3-dihydroxy-6-nitro-7-sulfamoylbenzoquionxaline, picrotoxin, or AP5^[3].
Ro 67-7476 activates ERK1/2 phosphorylation in the absence of exogenously added glutamate (EC₅₀=163.3 nM). The EC50 value of full P-ERK1/2 activation for Ro 67-7476 are nearly identical to the EC₅₀ for calcium mobilization potentiation^[3].
Ro 67-7476 increases basal cAMP production approximately by 8%. It potentiated threshold responses to glutamate in the cAMP accumulation assay, with an EC₅₀ value of 17.7 uM^[3].