Hemin

16009-13-5

C34H32ClFeN4O4

DMSO : 17.33 mg/mL (26.58 mM; Need ultrasonic)

Autophagy; Autophagy

Hemin is an iron-containing porphyrin. Hemin is an Heme oxygenase (HO)-1 inducer. IC50 & Target: Heme oxygenase^[1] In Vitro: Hemin and PGJ2, used as positive controls, strongly increase both expression and activity of HMOX after 4 and 12 h, respectively. Indeed, a significant effect is found of 30 uM Hemin on cell proliferation in all used cell lines after 48 h, which is dose-dependent. Hemin treatment decreases cell proliferation to 62+/-5 %, 51+/-3 %, and 38+/-8 % in PA-TU-8902, BxPC-3 and MiaPaCa-2 cancer cells, respectively, with p<0.0001 for all comparisons. Furthermore, enhancement of anti-proliferative effects of statins is observed by Hemin, documented as decreased cell proliferation after 48 h of co-treatment^[1]. In Vivo: Following the i.p. administration of Hemin (100 umol/kg), the HO-1 level in the renal cortex begins to increase gradually. The HO-1 level reaches its peak 24 h after Hemin preconditioning. HO-1 is expressed mainly in the renal tubules. The HO-2 level in the kidney does not change following Hemin preconditioning^[2].