Isoorientin

4261-42-1

C21H20O11

DMSO : >= 100 mg/mL (223.03 mM)

Immunology/Inflammation

Isoorientin is a potent inhibitor of COX-2 with an IC₅₀ value of 39 uM. IC50 & Target: IC50: 39 uM (COX-2)^[1] In Vitro: Isoorientin is a Selective Inhibitor of Cyclooxygenase-2 (COX-2) from the Tubers of Pueraria tuberosa^[1]. PANC-1 and PATU-8988 cells are grown for 24 hours in the presence of Isoorientin (0, 20, 40, 80, and 160 uM), and a CCK8 solution is added. The cell viability decreases significantly at the concentrations of 20, 40, 80, and 160 uM. After the cells are cultured with Isoorientin (0, 20, 40, 80, and 160 uM for PANC-1; 0, 20, 40, 80, 160, and 320 uM for PATU-8988) for 24 hours, the expression of p-AMPK and AMPK is assessed by Western blotting. After the Isoorientin treatment, the p-AMPK expression is increased. Then, in the shRNA group, the concentration of 80 uM is used to detect the effects of Isoorientin. The expression levels of AMPK and p-AMPK are much lower in the shRNA group than in the wild-type PC cells (WT) and the group that is transfected with a negative control lentivirus (NC)^[2]. In Vivo: Animals treated with Isoorientin at 10?mg/kg and 20?mg/kg body weight have a statistically significant reduction in paw edema, with a mean peak thickness of 1.19+/-0.05?mm and 1.08+/-0.04?mm, respectively. This indicated that Isoorientin significantly attenuates paw edema compared with the control group^[3].