Cresyl Violet acetate

10510-54-0

C18H15N3O3

DMSO : 1 mg/mL (3.11 mM; Need ultrasonic); H2O : 0.67 mg/mL (2.09 mM; Need ultrasonic)

Others

Cresyl Violet acetate is a red fluorescent stain, which can be used to stain neurons. In Vitro: The estimated total number of SG neurons is 27, 485+/-3251 and 26, 705+/-1823 in the PV and Cresyl Violet acetate stained sections, respectively. There is no significant difference between them (p=0.552). Therefore, Cresyl Violet acetate staining is simpler and more cost effective when estimates neuronal number. Although PV stains spiral ganglion neurons (SGNs) with a greater intensity and provides a functional status, its tedious protocol limits its use for quantification. Total RC volume is estimated using probe and it is found that an average RC volume of 2.162+/-0.35 mm³ and 1.82+/-0.33 mm³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Volume of neurons is estimated using nucleator probe and it is 3487.63+/-951 um³ and 3740.1+/-784 um³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively. Similarly, volume of neuronal nucleus is also estimated using nucleator probe and it is found to be 131.68+/-50 um³ and 126.51+/-33 um³ in Cresyl Violet acetate staining and PV immunostaining sections, respectively^[1].