740 Y-P

1236188-16-1

C141H222N43O39PS3

H2O : 20 mg/mL (6.11 mM; Need ultrasonic)

PI3K/Akt/mTOR

740 Y-P (PDGFR 740Y-P) is a potent and cell permeable PI3K activator. IC50 & Target: PI3K^[1] In Vitro: 740 Y-P is a synthetic biotinylated peptide consisting of the 16-amino acid sequence derived from the third helix of the Antennapedia protein in tandem with a 9 amino acid phosphopeptide mimetic of the high affinity p85 binding site on the PDGF receptor. 740 Y-P readily binds GST fusion proteins containing both the N- and C- terminal SH2 domains of p85. 740 Y-P fails to bind GST alone, or a GST fusion protein containing the N- terminal SH2 domain of PLCg and shows relatively weak binding of a GST fusion proteins containing the SH2 domain of Grb2. The 740 Y-P peptide is also able to specifically interact with p85 in cell lysates as shown by affinity precipitation. 740 Y-P stimulates mitogenesis at the lowest concentration tested (1 mg/mL). The peptide stimulates mitogenesis in both the presence and absence of serum (0.5%), and in the former instance a maximal response observed at 50 mg/mL. 740Y-P peptide to stimulate mitogenesis is highly specific and not a general feature of a cell permeable SH2 domain binding peptides^[1]. In Vivo: 740 Y-P is not only internalised in living cells but can also interact with p85 in vivo.^[1]