Comparison

Recombinant E.coli glk protein

Item no. NKM-ATGP3233-50ug
Manufacturer NKMAX
Amount 50 ug
Quantity options 100 ug 10 ug 20 ug 250 ug 4 ug 500 ug 50 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against Escherichia coli (E.coli)
Host E.coli
Conjugate/Tag HIS
Purity > 95% by SDS-PAGE
Sequence <MGSSHHHHHH SSGLVPRGSH MGS>MTKYALV GDVGGTNARL ALCDIASGEI SQAKTYSGLD YPSLEAVIRV YLEEHKVEVK DGCIAIACPI TGDWVAMTNH TWAFSIAEMK KNLGFSHLEI INDFTAVSMA IPMLKKEHLI QFGGAEPVEG KPIAVYGAGT GLGVAHLVHV DKRWVSLPGE GGHVDFAPNS EEEAIILEIL RAEIGHVSAE RVLSGPGLVN LYRAIVKADN
Citations Kawai S, Mukai T, et al. (2005) J. Biosci. Bioeng. 99 (4): 320-30.
NCBI NP_416889
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias Glucokinase,ECK2384,JW2385
Similar products Glucokinase, glk, ECK2384, JW2385, ATGP3233-10ug, ATGP3233-20ug, ATGP3233-50ug, ATGP3233-100ug, ATGP3233-250ug, ATGP3233-500ug, ATGP3233-1mg, ATGP3233-10, ATGP3233-20, ATGP3233-50, ATGP3233-100, ATGP3233-250, ATGP3233-500, ATGP3233-1
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Enzyme Activity
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
37.1 kDa (344aa) confirmed by MALDI-TOF
Description
glk belongs to the bacterial glucokinase family. This protein is not highly important in E. coli as glucose is transported into the cell by the PTS system already as glucose 6-phosphate. Recombinant E. coli glk protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.
Concentration
1mg/ml (determined by Bradford assay)
Formulation
Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol
Antigen Species
E.coli
Gene Category
Metabolism
Domain
1-321aa
Bioactivity Comment Only
Specific activity is > 70unit/mg obtained by measuring the increase of NADPH in absorbance at 340 nm resulting from the reduction of NADP. One unit will oxidize 1.0 umole of Glucose to D-glucose 6-phosphate per minute in the presence of Beta-NADP at pH 9.0 at 37C.
Species of Protein
E.coli
Manufacturer - Search Terms
glk, Glucokinase, ECK2384, JW2385, ATGP3233-10ug, ATGP3233-20ug, ATGP3233-50ug, ATGP3233-100ug, ATGP3233-250ug, ATGP3233-500ug, ATGP3233-1mg, ATGP3233-10, ATGP3233-20, ATGP3233-50, ATGP3233-100, ATGP3233-250, ATGP3233-500, ATGP3233-1

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
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