ichorGreen Nucleic Acid Gel Stain (10,000x in DMSO)

ichorGreen Nucleic Acid Gel Stain is the third generation non-mutagenic fluorescent nucleic acid gel stain to replace the SYBR Green (SG) or the expensive GelGreen (GG) from Biotium for staining dsDNA, ssDNA or RNA in agarose gels or polyacrylamide gels. ichorGreen is more sensitive than SG and GR either as precast gel stains or post gel stains. We can directly replace SG or GG with ichorGreen without changing the existing imaging system since they all have the similar spectra. ichorGreen can also be used to stain dsDNA, ssDNA or RNA in polyacrylamide gel via post gel staining. Gel staining with ichorGreen is compatible with downstream applications such as gel extraction and cloning. ichorGreen is efficiently removed from DNA by phenol/chloroform extraction and ethanol precipitation.

ichorGreen at 3x concentration is classified as nonhazardous to aquatic life, under CCR Title 22 regulation. ichorGreen at 3x or lower concentrations can be safely released into the environment.

Dilute the ichorGreen 10, 000X stock reagentca.3, 300 fold to make a 3X staining solution in H2O. Note: including 0.1M NaCl in the staining solution enhances sensitivity, but may promote dye precipitation if the gel stain is reused.Carefully place the gel in a suitable polypropylene container. Gently add a sufficient amount of the 3X staining solution to submerge the gel.Agitate the gel gently at room temperature for 30 minutes. ichorGreen: Image the stained gel with a 254nm transilluminator, a Dark Reader or a similar transilluminator, or a laser-based gel scanner using a long path green filter such as a SYBR filter or GelStar filter. Staining solution can be resued 2-3 times. Store staining solution at room temperature protected from light

Room temperature protected from light for at least one year