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EasyScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)&

EasyScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR (One-Step gDNA Removal)&

Item no.	TRG-AE341-02
Manufacturer	TransGen Biotech
Amount	100 rxns x 20 ul system
Quantity options	100 rxns x 20 ul system 500 rxns x 20 ul system
Category	Nucleic Acids DNA cDNA
Type	Reagents
Specific against	other
Dry ice	Yes
Citations	1 He F, Cheng K, Qi J, et al. Black phosphorus nanosheets enhance differentiation of neural progenitor cells for improved treatment in spinal cord injury[J]. Chemical Engineering Journal, 2023.(IF 15.10) 2 Liu X, Hou S, Xiang R, et al. Imipramine activates FAM3A-FOXA2-CPT2 pathway to ameliorate hepatic steatosis[J]. Metabolism, 2022.(IF 13.93) 3 He F, Liu Z, Xu J, et al. Black phosphorus nanosheets suppress oxidative damage of stem cells for improved neurological recovery[J]. Chemical Engineering Journal, 2023.(IF 16.74) 4 Yin Q, Li Y, Zhou Z, et al. RPA1 controls chromatin architecture and maintains lipid metabolic homeostasis[J]. Cell Reports, 2022.(IF 9.99) 5 Zhang Y, Zhang M, Yao A, et al. Circ_0011129 Encapsulated by the Small Extracellular Vesicles Derived from Human Stem Cells Ameliorate Skin Photoaging[J]. International Journal of Molecular Sciences, 2022.(IF 6.20)
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12000000
Available
Manufacturer - Applications	High-copy-number and low-copy number gene detection
Manufacturer - Category	RT-PCR
Storage Conditions	at -20 °C for two years
Product Details	5×EasyScript® All-in-One SuperMix for qPCR provides all the necessary reagents for cDNA synthesis from total RNA or mRNA (including 5×EasyScript® RT, RNase Inhibitor, Anchored Oligo (dT)18 Primer, Random Primer (N9), dNTPs, Buffer). It is provided at 5× concentration and used at 1× concentration by only adding gDNA remover, RNA template and H2O for efficient first-strand cDNA synthesis. Simultaneously, residual genomic DNA from RNA template can be removed. 5×EasyScript® All-in-One No-RT Control SuperMix for qPCR is supplied to prepare no-reverse transcriptase (RT) control, which is used to assess if the qPCR template is contaminated with genomic DNA. This product is capable of minimizing contamination during operation with a simple workflow. The resulting cDNA is only suitable for qPCR, not for regular PCR. Highlights • “All-in-One SuperMix” type: Simultaneous cDNA synthesis and genomic DNA removal by only adding gDNA remover, RNA template and H2O • High synthesis efficiency enabled by optimal ratio of Oligo(dT)18 Primer to Random Primer (N9) and optimized composition of the SuperMix, ensuring same reverse transcription efficiency for RNA templates of different concentrations and specifically high synthesis efficiency for short cDNA. • Only 15 minutes for reverse transcription. • High compatibility with qPCR reagents. Applications High-copy-number and low-copy number gene detection Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.