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TransTaq® DNA Polymerase High Fidelity (HiFi) (with 2.5 mM dNTPs)

TransTaq® DNA Polymerase High Fidelity (HiFi) (with 2.5 mM dNTPs)

Item no.	TRG-AP131-11
Manufacturer	TransGen Biotech
Amount	250 units
Quantity options	250 units 500 units 6 x 500 units 250 units 500 units 6 x 500 units
Category	Nucleic Acids DNA cDNA
Type	Enzymes
Specific against	other
Dry ice	Yes
Citations	1 Cao J, Huang C, Liu J, et al. Comparative Genomics and Functional Studies of Putative m6A Methyltransferase (METTL) Genes in Cotton[J]. International Journal of Molecular Sciences, 2022.(IF 6.208) 2 Du J, Wang X, Li Y, et al. DHA exhibits synergistic therapeutic efficacy with cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via modulation of iron metabolism[J]. Cell Death & Disease, 2021.(IF 8.40) 3 Di Y Q, Han X L, Kang X L, et al. Autophagy triggers CTSD (cathepsin D) maturation and localization inside cells to promote apoptosis[J]. Autophagy, 2021.(IF 9.77) 4 Li S, Li X, Xue W, et al. Screening for functional circular RNAs using the CRISPR–Cas13 system[J]. Nature methods, 2021.(IF 30.82)
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Available
Manufacturer - Category	PCR Enzyme
Storage Conditions	at -20 °C for two years
Product Details	TransTaq® DNA Polymerase High Fidelity (TransTaq®HiFi DNA Polymerase) contains TransTaq®-T DNA Polymerase and a proofreading 3’-5’ exonuclease.TransTaq® HiFi DNA Polymerase provides higher specificity and higher amplification efficiency than TransTaq®-T DNA Polymerase. Two different buffers are provided in the kit.TransTaq® HiFi Buffer I is optimized for the amplification of genomic DNA and TransTaq® HiFi Buffer II is optimized for the amplification of λDNA, cDNA or plasmid DNA. • TransTaq® HiFi DNA Polymerase offers 18-fold fidelity as compared to EasyTaq® DNA Polymerase. • Extension rate is about 1-2 kb/min. • Template-independent “A” can be generated at the 3’ end of the PCR product. PCR products can be directly cloned into pEASY®-T vectors. • Amplification of genomic DNA fragment up to 15 kb. Applications • Complex templates • GC/AT rich templates • Long PCR • High yield PCR Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.