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2×TransStart® FastPfu PCR SuperMix (-dye)

2×TransStart® FastPfu PCR SuperMix (-dye)

Item no.	TRG-AS221-01
Manufacturer	TransGen Biotech
Amount	1 ml
Quantity options	1 ml 5 x 1 ml
Category	Nucleic Acids DNA cDNA
Type	Reagents
Specific against	other
Dry ice	Yes
Citations	1 Song R, Guo P, Ren X, et al. A novel polypeptide CAPG-171aa encoded by circCAPG plays a critical role in triple-negative breast cancer[J]. Molecular Cancer, 2023.(IF 37.30) 2 Jin S, Lin Q, Gao Q, et al. Optimized prime editing in monocot plants using PlantPegDesigner and engineered plant prime editors (ePPEs)[J]. Nature Protocols, 2022.(IF 17.02) 3 Chen K, Hu Z, Song W, et al. Diversity of O-glycosyltransferases contributes to the biosynthesis of flavonoid and triterpenoid glycosides in Glycyrrhiza uralensis[J]. ACS Synthetic Biology, 2019.(IF 5.57) 4 Wang Y, Wang Z, Chen Y, et al. A highly efficient CRISPR-Cas9-based genome engineering platform in Acinetobacter baumannii to understand the H2O2-sensing mechanism of OxyR[J]. Cell Chemical Biology, 2019.(IF 6.76)
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12000000
Available
Manufacturer - Category	PCR SuperMix
Storage Conditions	at -20 °C for two years
Product Details	TransStart® FastPfu PCR SuperMix is a ready-to-use mixture of TransStart® FastPfu DNA polymerase, dNTPs, and optimized buffer, featuring high amplification efficiency, fast amplification speed, high fidelity and high specificity. The SuperMix is provided at 2× concentration and can be used at 1× concentration by adding template, primers and H2 O for amplification. The amplified product of 2×TransStart® FastPfu PCR SuperMix is blunt-ended and can be cloned directly into pEASY®-Blunt series of vectors. It can also be directly loaded on agarose gel for electrophoresis. If it is used for cloning, it needs to be purified to remove the dye. Its PCR product is not suitable for polyacrylamide gel electrophoresis. • Reduce PCR operation time. • Avoid contamination caused by the multi-step operation. • TransStart® FastPfu PCR SuperMix offers 54-fold fidelity as compared to EasyTaq® DNA Polymerase. • Amplification of genomic DNA fragment up to 15 kb. • Amplification of plasmid DNA fragment up to 20 kb. Applications • High fidelity PCR • Site-directed mutagenesis • Blunt end cloning • Complex templates • Amplification of templates with high GC/AT content • Long fragment amplification Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.