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TransScript® Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

TransScript® Uni One-Step gDNA Removal and cDNA Synthesis SuperMix

Item no.	TRG-AU311-03
Manufacturer	TransGen Biotech
Amount	100 rxns x 20 ul syetem
Quantity options	50 rxns x 20 ul system 100 rxns x 20 ul syetem
Category	Nucleic Acids DNA cDNA
Type	Reagents
Specific against	other
Dry ice	Yes
Citations	1 Li C, Guan H, Jing X, et al. Genomic insights into historical improvement of heterotic groups during modern hybrid maize breeding[J]. Nature Plants, 2022.(IF 17.35) 2 Chen L, Zhang C, Ma W, et al. METTL3-mediated m6A modification stabilizes TERRA and maintains telomere stability[J]. Nucleic Acids Research, 2022.(IF 19.16) 3 Wang Y, Gong Q, Wu Y, et al. A calmodulin-binding transcription factor links calcium signaling to antiviral RNAi defense in plants[J]. Cell Host & Microbe, 2021.(IF 21.02) 4 Kumar S, Wang G, Liu W, et al. Hypoxia-induced mitogenic factor promotes cardiac hypertrophy via calcium-dependent and hypoxia-inducible factor-1α mechanisms[J]. Hypertension, 2018.(IF 6.82)
ECLASS 10.1	42051003
ECLASS 11.0	42051003
UNSPSC	12000000
Available
Manufacturer - Category	RT-PCR
Storage Conditions	at -20 °C for two years
Product Details	TransScript®-Uni RT is an improved version of M-MLV reverse transcriptase with broad range of reaction temperature(42°C-65°C) and higher thermostability. The suggested reaction temperature is 50°C. The SuperMix contains reagents for simultaneous genomic DNA removal and cDNA synthesis. After cDNA synthesis, gDNA remover and reverse transcriptase are inactivated by heating at 85°C for 5 seconds. • Broad range reaction temperature (42°C -65°C ) . • Simultaneous genomic DNA removal and cDNA synthesis in one tube to minimize RNA contamination. • The product obtained from 15 minutes reaction is used for qPCR; the product obtained from 30 minutes reaction is used for PCR. • cDNA up to 20 kb. Applications • Multiple copy and low copy gene detection • GC-rich or complex secondary structure RNA template • cDNA library construction, primer extension, 3’ and 5’ RACE Storage at -20 ℃ for two years Shipping Dry ice (-70 ℃)

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.