Plasmid #33

Plasmid #34 enables target proteins to be labeled with C-terminal His8 and CL7. Protease cleavage removes both tags. EXPRESSION: Transcription is induced with IPTG and driven by the T7 RNA polymerase. The plasmid is designed for expression in E. coli. PEPTIDE TAG The C-terminal CL7 tag is downstream of Sortase A (SRT) and PreScission protease (PSC) cleavage sites and a His8 tag. CLEAVAGE SITE(S) N-terminal SUMO and C-terminal SRT and PSC cleavage sites are available in Plasmid #34. OTHER TAGS: The encoded region begins with a Trx tag and includes a C-terminal His8 tag upstream of CL7. CLONING OPTIONS:1. HindIII/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7 This insertion option results in 7 extra aa at the N terminus of the target following SUMO protease cleavage. 2. KpnI/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7 This insertion option results in 2 extra aa at the N terminus of the target following SUMO protease cleavage. 3. Bsu36I/SpeI Insertion Site – Trx | SUMO | Gene of Interest | SRT | PSC | PSC | PSC | His8 | CL7 EXPIRATION: 6 months from receipt when stored as directed. Plasmid guide with plasmid sequence file is available at Trialtusbioscience.com.