ExKine™ Pro Total Protein Extraction Kit for Plant Tissues

Organelle extraction and staining

Cell analysis

RT

ExKine™ Pro Plant Tissue Total Protein Extraction Kit (column method) is a new generation of ultra-fast protein extraction tool. More and more evidences show that the most commonly used RIPA buffer may lead to random loss of protein, resulting in many difficult data that are difficult to explain. ExKine™ Pro Total Protein Extraction Kit uses centrifugal column extraction technology combined with optimized lysis buffer to extract total protein more quickly and effectively, without grinding in liquid nitrogen, making WB results more accurate.

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

1. Protease inhibitors are not necessarily added, but if downstream experiments take longer or are preserved for longer periods after protein extraction, it is advisable to add protease inhibitors. Protein concentration was determined by BCA method, but not by Bradford method. To study protein phosphorylation, phosphatase inhibitors should be added to the lysis buffer before use.
2. Before WB, still mix the sample and loading buffer to boiling.
3. The Plastic Rod is reusable, rinse thoroughly with deionized water, and dry it with paper towel.
4. If the protein concentration is low, increase the weight of tissues or reduce the volume of Denaturing Cell Lysis Buffer.
5. Partial tissues that is not completely lysed will not affect the quality of the sample.

WB, SDS-PAGE