Comparison

CheKine™ Micro Proline (PRO) Assay Kit

Item no. ABK-KTB1430-48T
Manufacturer Abbkine Scientific
Amount 48 T
Quantity options 48 T 96 T
Category
Type Assay-Kit
Specific against other
Conjugate/Tag Pro
ECLASS 10.1 32161090
ECLASS 11.0 32161090
UNSPSC 41116126
Shipping Condition Cool pack
Available
Manufacturer - Type
Cell metabolism detection kit
Manufacturer - Applications
The kit provides a simple method for detecting Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria. In the assay, Pro is extracted with sulfosalicylic acid (SA). After heat treatment, Pro reacts with acidic ninhydrin solution to produce red; after extraction with toluene, the absorbance is measured at 520 nm.
Manufacturer - Category
Cell analysis
Shipping Temperature
Gel pack with blue ice.
Storage Conditions
Storage at 4°C and protected from light upon receipt. Kit has a storage time of 12 months form receipt.
Background
Pro is widely present in animals, plants, microorganisms and cultured cells. Under adversity conditions, the Pro content in plants increased significantly. The increased amount of Pro reflects stress resistance to a certain extent, and varieties with strong drought resistance tend to accumulate more proline. Therefore, the increased amount of proline can be used as one of the physiological indicators of stress resistance breeding.
Description
Abbkine CheKine™ Proline (PRO) Colorimetric Assay Kit is designed for detecting Pro content in the sample.
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
• Determination of Pro content in a variety of biological samples such as serum, plasma, Plant or animal tissue, cells, and bacteria.
• A broad range linearity: 0.5-80 μg/mL.
• Detailed sample preparation and result calculation methods are provided.
• Simple operation steps.
• Standard and standard curve line are provided.
Usage Notes
• 96 Well Clear Flat Bottom UV-Transparent Microplate is needed.
• Do not mix or substitute reagents or materials from other kit lots or vendors.
• Avoid foaming or bubbles when mixing or reconstituting components.
• Avoid cross-contamination, change pipette tips between additions of standards, samples and reagents. Also, use separate reservoirs for each reagent.
• Ensure all reagents and equipment are at the appropriate temperature before starting the assay.
Kit components
• Extraction Buffer
• Assay Buffer I
• Chromogen
• Pro Substrate
Limit of detection
0.5 μg/mL
Calibration range
0.5-80 μg/mL

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 48 T
Available: In stock
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