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Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Choline Acetyltransferase (ChAT) Activity Assay Kit (Tissue Samples)

Item no.	E-BC-K125-M-500Assays
Manufacturer	Elabscience
Amount	500 Assays
Quantity options	48 T 500 Assays 96 T
Category	Neuroscience Molecular Targets for Neuroscience Neural Signaling Other Assays & Kits Other Kits
Type	Kit
Specific against	other
Sensitivity	1.21 U/g fresh weigh
Citations	https://www.mdpi.com/2050368
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Alias	ChAT
Shipping Condition	Cool pack
Hazard information	Comply with cargo safety certificate
Available
Manufacturer - Category	Cell Metabolism Assays
Shipping Temperature	Ice bag
Storage Conditions	This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
Research Areas	Amino Acids and Proteins
Description	Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
Assay time	65 min
Detection Type	Colorimetric method
Tested Sample Types	animal tissue
Detection Instrument	Microplate reader
Test Principle	Acetyl-CoA can react with choline under the catalysis of choline acetyltransferase (ChAT) to produce coenzyme A (CoA), CoA can combine with the 4, 4-dithiopyridine. The activity of ChAT can be calculated indirectly by measuring the OD value at 324 nm.
Result Type	Enzyme Activity
Inter cv	9.4%
Intra cv	5.1%
Category ID III	Amino Acids and Proteins

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.