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PUREfrex 1.0

PUREfrex 1.0

Item no.	GFK-PF001-50ML-EX
Manufacturer	Cosmobio
Amount	25 x 2 ml
Quantity options	250 ul 5 x 2 ml 2 ml 25 x 2 ml
Category	Other Assays & Kits Other Kits
Type	Kit
Specific against	other
ECLASS 10.1	32161090
ECLASS 11.0	32161090
UNSPSC	41116126
Available
Manufacturer - Category	Protein Engineering/Cell Free Synthesis/Reconstituted/Coupled Transcription & Translation Kits
Maker	GFK
Description	PUREfrex® (manufactured by Gene Frontier Corporation) is an E. coli reconstituted coupled transcription/translation system based on the PURE system originally developed by the Ueda group (Shimizu et al., 2001) and later commercialized as PURESYSTEM® (Shimizu and Ueda, 2010) by BioComber (Tokyo Japan). PUREfrex® contains highly purified E. coli proteins comprising modules for aminoacylation, energy regeneration, transcription and translation. Among the many advantages of PUREfrex® over competing coupled transcription/translation systems are reduced levels of contaminating proteases, nucleases, and phosphatases, beta-galactosidase and LPS. Compared to cell lysate-based systems, the more defined and modular chemistry of PUREfrex® leads to greater yield, reproducibility and flexibility. The untagged protein components of PUREfrex® (in contrast to the hexahistidine-tagged components of BioComber' s PURESYSTEM® and New England Biolab' s PURExpress® system) permit addition of any desired tag (including His-tags) to a protein of interest and subsequent rapid and simple purification by a single immobilized metal affinity chromatography step. Protein yields can reach 600ng/ul. PUREfrex® is available in three forms: PUREfrex® 1.0, PUREfrex® 2.0 and PUREfrex® 2.1. PUREfrex® can be used to express proteins containing non-natural amino acids by exploiting Protein Express' s CloverDirect™ line of tRNA reagents for site-directed protein functionalization. CloverDirect™ UAG or CGGG aminoacyl tRNAs are available charged with different non-natural amino acids that confer a range of functionality, including fluorescent-labeling, biotin-labeling, PEGylation, chemical crosslinking and photo-regulation. Targeting incorporation of specific non-natural amino acids is achieved by designing protein expression templates with UAG and/or CGGG codons at user-defined positions. For template construction, we recommend Protein Express Linear Template PCR kits (available in two forms: His-tag only or ProX-tag + His-tag) or expression plasmids from Protein Express (pROX-FL92.1: ProX-tag + His-tag) or BioComber (pURE1-4: no tags). The ProX tag is a special N-terminal peptide designed for the incorporation of certain large unnatural amino acids that require precise N-terminal positioning (codon 9) and a specific codon context (flanked by AAC and TCT). Codon 9 of the ProX tag can be varied between UAG, CGGG or TTT (as a control).

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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Amount: 25 x 2 ml

available

Delivery expected until 8/14/2025

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