PuroMAG™ Endo-Free Plasmid MIDIprep Kit, Magnetic

The PuroMAG™ Endo-Free Plasmid MIDIprep Kit is designed to provide a simple and easy-to-use method for large-scale extraction and purification of endotoxin-free plasmid DNA from cultured bacterial cells.

This purification technology is based on plasmid binding to silica-coated magnetic beads, which allows for efficient removal of genomic DNA, protein, salt, and other contaminants. Following the lysis steps, endotoxins are removed using the Endotoxin Removal Buffer ERB.
Silica coated magnetic beads provide the fastest and simplest method for the extraction of plasmid DNA on a MIDI scale compared to gravity flow ion exchange of silica spin column kits. With short 2 minute magnetic separation steps, the  protocol can be completed in under 30 minutes. Bead binding and washing steps are all performed on a magnetic rack without the need for centrifugation.

Endo-Free Plasmid MIDIprep Kit can be used to purify plasmid DNA from up to 100 mL of overnight bacterial culture. PuroMAG™ Endo-Free Plasmid MIDIprep Kit can purify up to 200 µg of plasmid DNA while keeping endotoxin levels at <0.1 EU/µg.

The PuroMAG™ buffer formulations produce highly purified plasmid DNA for downstream applications, such as transfection, enzymatic digestion, or PCR.