TransDirect® Plant Tissue PCR Kit

TransDirect® Plant Tissue PCR Kit uses a unique lysis buffer to lyse plant tissues (fresh or frozen). The resulting lysate without purification can be directly used as PCR template. 2×TransDirect® PCR SuperMix (+dye) is highly resistant to various PCR inhibitors present in plant tissues. PCR product can be directly used for gel electrophoresis.


• No DNA extraction needed.
• Ready-to-use SuperMix.
• DNA fragment up to 2 kb.
• For plants with moderate contents of polysaccharides or polyphenols.


Applications
• Direct amplification from unpurified lysate. Suitable for high throughput applications.
• Amplification of genomic DNA fragment up to 2 kb.


Storage
2×TransDirect® PCR SuperMix (+dye) at -20°C for two years, others at room temperature (15-25°C) for two years.


Shipping
2×TransDirect® PCR SuperMix (+dye) on dry ice (-70 ℃); others at room temperature.