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PNGase F

PNGase F

Item no.	ANSY-YXX04901-75KU
Manufacturer	AntibodySystem
Amount	75 KU
Quantity options	15 KU 75 KU
Category	Peptides & Proteins Enzymes
Type	Enzymes
Format	Liquid
Specific against	Bacteria (generic)
Purity	>95% as determined by SDS-PAGE.
ECLASS 10.1	32160410
ECLASS 11.0	32160410
UNSPSC	12352204
Available
Specificity	Elizabethkingia miricola
Manufacturer - Applications	Removal of high mannose N-glycans from glycoproteins
Storage Conditions	Use a manual defrost freezer and avoid repeated freeze thaw cycles.Store at 2 to 8 °C for one week .Store at -20 to -80 °C for twelve months from the date of receipt.
Molecular Weight	37.08 kDa
Product description	Peptide -N-Glycosidase F, also known as PNGase F, is an amidase that cleaves between the innermost GlcNAc and asparagine residues of high mannose, hybrid, and complex oligosaccharides from N-linked glycoproteins
Storage buffer	20mM Tris-HCl, pH 7.5, 50mM NaCl, 5mM EDTA, 50% glycerol
Endotoxin level	Please contact with the lab for this information.
Note	For research use only.
Expression System	E. coli
Biological Activity	One unit is defined as the amount of enzyme required to remove > 95% of the carbohydrate from 10 μg of denatured RNase B in 1 hour at 37°C in a total reaction volume of 10 μl.
Protein Fragment	PNGase F is cloned from Elizabethkingia miricola and expressed in E.coli.

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

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