Comparison

Recombinant mouse 5′-Nucleotidase/CD73 protein

Item no. NKM-ATGP3509-50ug
Manufacturer NKMAX
Amount 50 ug
Quantity options 100 ug 10 ug 20 ug 250 ug 500 ug 50 ug 5 ug
Category
Type Proteins Recombinant
Format Liquid
Applications SDS-PAGE
Specific against Mouse (Murine, Mus musculus)
Host Baculovirus-Infected Insect Cells
Conjugate/Tag HIS
Purity > 90% by SDS-PAGE
Sequence WELTILHTND VHSRLEQTSD DSTKCLNASL CVGGVARLFT KVQQIRKEEP NVLFLDAGDQ YQGTIWFTVY KGLEVAHFMN ILGYDAMALG NHEFDNGVEG LIDPLLRNVK FPILSANIKA RGPLAHQISG LFLPSKVLSV GGEVVGIVGY TSKETPFLSN PGTNLVFEDE ISALQPEVDK LKTLNVNKII ALGHSGFEMD KLIAQKVRGV DIVVGGHSNT FLYTGNPPSK EV
Citations Koszalka P., et al. (2016) PLoS One. 11:e0151420.
Braun N., et al. (1995) Brain Res Dev Brain Res. 88:79-86.
NCBI NP_035981.1
ECLASS 10.1 32160409
ECLASS 11.0 32160409
UNSPSC 12352202
Alias CD73,5-NT,5'-nucleotidase,5'-nucleotidase ecto,AI447961,E5NT,ecto,eN,eNT,NT,NT5,Nt5e,NTE,2210401F01Rik
Similar products NT5E, NTE, NT5, 5'-nucleotidase, CD73, NT, eN, eNT, CALJA, 5'-NT, 5-nucleotidase, 5-NT, Purine 5-Prime-Nucleotidase, 5' NT, 5' nucleotidase, ecto, AI447961, ecto-5'-nucleotidase, 5 nucleotidase, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5' nucleotidase ecto, 5 nucleotidase ecto, 2210401F01Rik, ATGP3509-10ug, ATGP3509-20ug, ATGP3509-50ug, ATGP3509-100ug, ATGP3509-250ug, ATGP3509-500ug, ATGP3509-1mg, ATGP3509-010, ATGP3509-020, ATGP3509-050, ATGP3509-100, ATGP3509-250, ATGP3509-500, ATGP3509-01M
Available
Manufacturer - Type
Recombinant Protein
Manufacturer - Applications
SDS-PAGE, Enzyme Activity
Manufacturer - Host
Baculovirus
Storage Conditions
Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.
Molecular Weight
59.1 kDa (531aa)
Description
Nt5e, also known as 5-nucleotidase, is the main enzyme responsible for conversion of AMP into the immunosuppressive molecule adenosine. It was demonstrated to play a direct role in tumor progression including regulation of tumor vascularization. It is of general functional importance for the metabolism of nucleotides at the ventricular surface of the retina as well as the ventricles of the brain, a feature that is maintained throughout development. Recombinant mouse Nt5e, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.
Concentration
0.5mg/ml (determined by absorbance at 280nm)
Formulation
Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol
Antigen Species
Mouse
Gene Category
Cardiovascular
Domain
29-551aa
Endotoxin
< 1 EU per 1ug of protein (determined by LAL method)
Bioactivity Comment Only
Specific activity is > 8, 000pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1.0pmole of Adenosine 5-monophosphate to phosphate per minute per minute at pH 7.5 at 25C.
Predicted nterm
Trp29
Species of Protein
Mouse
Manufacturer - Search Terms
Purine 5-Prime-Nucleotidase, NTE, NT5E, NT5, NT, eNT, eN, ecto-5'-nucleotidase, ecto, CD73, CALJA, AI447961, 5-nucleotidase ecto, 5'-nucleotidase ecto, 5-nucleotidase, 5'-nucleotidase, 5-NT, 5'-NT, 5' nucleotidase ecto, 5 nucleotidase ecto, 5' nucleotidase, 5 nucleotidase, 5' NT, 2210401F01Rik, ATGP3509-10ug, ATGP3509-20ug, ATGP3509-50ug, ATGP3509-100ug, ATGP3509-250ug, ATGP3509-500ug, ATGP3509-1mg, ATGP3509-010, ATGP3509-020, ATGP3509-050, ATGP3509-100, ATGP3509-250, ATGP3509-500, ATGP3509-01M

Note: The presented information and documents (Manual, Product Datasheet, Safety Datasheet and Certificate of Analysis) correspond to our latest update and should serve for orientational purpose only. We do not guarantee the topicality. We would kindly ask you to make a request for specific requirements, if necessary.

All products are intended for research use only (RUO). Not for human, veterinary or therapeutic use.

Amount: 50 ug
Available: In stock
available

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