[Novoprotein] Recombinant Human Ribosomal Protein S6 Kinase a-1/RPS6KA1/RSK1(C-6His)

Lyophilized protein should be stored at < -20C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20C for 3 months.

Recombinant Human Ribosomal protein S6 kinase alpha-1 is produced by our Mammalian expression system & the target gene encoding Met1-Leu735 is expressed with a 6His tag at the C-terminus.

Ribosomal protein S6 kinase alpha-1(RPS6KA1) contains 1 AGC-kinase C-terminal domain, contains 2 protein kinase domains & belongs to the protein kinase superfamily. In fibroblast, RPS6KA1 is required for EGF-stimulated phosphorylation of CREB1, which results in the subsequent transcriptional activation of several immediate-early genes. In response to mitogenic stimulation (EGF & PMA), It phosphorylates & activates NR4A1/NUR77 & ETV1/ER81 transcription factors & the cofactor CREBBP. Upon insulin-derived signal, it acts indirectly on the transcription regulation of several genes by phosphorylating GSK3B at 'Ser-9' & inhibiting its activity.

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 ug/ml.
Dissolve the lyophilized protein in ddH2O.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.