UBE2L3 Recombinant Protein (Human)

Protein

Dry Ice

Typical enzyme concentration to support conjugation in vitro is 100nM to 1uM depending upon conditions.

ubiquitin conjugating enzyme E2 L3

Liquid. In HEPES, pH 7.5, containing 150 mM NaCl and 1.0mM DTT.

Ubiquitin-conjugating enzyme E2 that specifically acts with HECT-type and RBR family E3 ubiquitin-protein ligases. Does not function with most RING-containing E3 ubiquitin-protein ligases because it lacks intrinsic E3-independent reactivity with lysine: in contrast, it has activity with the RBR family E3 enzymes, such as PRKN and ARIH1, that function like function like RING-HECT hybrids. Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.